ABSTRACT
The essential oil obtained by hydrodistillation from fresh leaves of Casearia lasiophylla Eichler, Salicaceae, was analyzed by gas capillary (GC/FID and GC/MS). The cytotoxicity of the leaves essential oil was tested in vitro againstU251 (glioma), UACC-62 (melanoma), MCF-7 (breast), NC1-ADR/RES (ovarian-resistant), NCI-H460 (lung), PC03 (prostate), OVCAR-3 (ovarian), HT-29 (colon) and K562 (leukemia) human cancer cells and against VERO (no cancer cell). The yield of oil was 0.02 percent. Fifty two compounds were identified, representing 87.1 percent of the total of the oil. The main components were identified as germacrene D (18.6 percent), β-caryophyllene (14.7 percent), δ-cadinene (6.2 percent), and α-cadinol (5.4 percent). The oil exhibited antiproliferative activity against all cell lines (TGI<100 µg/mL), with exception of NCI-H460 cell line (TGI 191.31 µg/mL). The highest activity was observed against UACC-62 (TGI 7.30 µg/mL), and K562 (TGI 7.56 µg/mL) cell lines. The observed activity could be related to high content of germacrene D and β-caryophyllene, compounds known as cytotoxic.
ABSTRACT
Os óleos essenciais, isolados por hidrodestilação de folhas e flores de Myrcia obtecta (O. Berg) Kiaersk. var. obtecta, Myrtaceae, coletadas a cada dois meses em Curitiba, Paraná, durante o período de outubro/2005 a agosto/2006, foram analisados por CG e CG/EM. Os óleos das folhas foram caracterizados pelo elevado conteúdo de sesquiterpenos cíclicos (cerca de 70 por cento) e quantidades variáveis de monoterpenos (5,6-16,7 por cento), compostos alifáticos (0,4-5,5 por cento) e salicilato de metila (0,1-1,5 por cento). Foram identificados 55 componentes, sendo que trans-calameneno (17,029,3 por cento) foi o componente majoritário em todas as amostras. Não houve mudanças significativas na composição, excepto em outubro (floração) quando α-terpineol e trans-calameneno atingiram os maiores níveis (11,2 por cento e 29,3 por cento, respectivamente). O óleo das flores contém salicilato de metila (89 por cento) como constituinte majoritário.
The essential oils isolated by hydrodistillation from leaves and flowers of Myrcia obtecta (O. Berg) Kiaersk. var. obtecta, Myrtaceae, collected bimonthly in Curitiba, Paraná state, Brazil, from October/2005 to August/2006 were analyzed by capillary GC and GC/MS. Fifty-five components were identified in the leaf oils, which were characterized by predominance of cyclic sesquiterpenes (around 70 percent) and variable amounts of monoterpenes (5.616.7 percent), aliphatic compounds (0.4-5.5 percent) and methyl salicylate (0.1-1.5 percent). The major component in all samples was trans-calamenene (17.0-29.3 percent). The composition did not show significant seasonal variation, except in October (flowering) when α-terpineol and trans-calamenene peaked (11.2 percent and 29.3 percent, respectively). The flower oil was characterized by high content of methyl salicylate (89 percent).